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/histologie/technovit-9100-image-640w-360h.jpg "Technovit® 9100")
Technovit® 9100
For Sections and Cuts
Technovit 9100 is the specialist for the embedding of mineralised tissues as well as soft tissue with an expanded study spectrum in light microscopy. Technovit 9100 is a plastic embedding system based on MMA (Methyl methracrylate).
Technovit 9100 is used for example in medicine, botany and zoology. The deplasticized sections are suitable for histological overview staining, enzyme chemistry and immunohistological studies, including in-situ hybridisation. Polymerisation of the hydrophobic Technovit 9100 occurs by excluding oxygen using a catalyst system made of peroxide and amine. Additional components such as PMMA powder and regulator allow for a controlled polymerisation in the cold (in the range of -2 to -20 °C, depending on the volume) that guarantees complete dissipation of the polymerisation heat.
Benefits
- Polymerisation below freezing
- Reproducibility of the embedding results and reliability due to constant, documented quality controls
- Equal block hardening
- The PMMA block remains transparent
- Better results with regard to cutting and staining because Technovit® 9100 contains a hydrophilising agent
- Can be used for thin section and the sawing and cutting techniques
- Enzyme histology and immunohistology as well as in-situ hybridisation possible (sections and cuts)
Applications
- Hard-cutting technique for making thin layers
Examples: Iliac crest biopsies, smaller, spongy and compact bone tissue specimens - Division thin section technique (division procedure in point contact technology)
Examples: Tooth/jaw areas with and without implants, non-cemented endoprostheses with shaft bones - Combined division-thin section technique and hard-cutting technique (target preparation)
Examples: Boundary layer and environment assessment for metal implants and non-cemented endoprostheses
Products
Technovit® 9100 Baseliquid - component (1)
The Technovit 9100 baseliquid is comprised of stablised methyl methacrylate. The hydrophily is improved through the addition of a suitable hydrophily agent. Technovit 9100 basic solution can be used when stabilised and unstabilised.
Technovit 9100 PMMA powder - component (2)
The PMMA powder is used to guarantee a clear decrease in polymerisation shrinkage, a reduction in the polymerisation heat released and a better polymerisation process.
Technovit 9100 hardener - component (3)
Hardening powder 1 is a peroxide compound that starts polymerisation with hardener 2.
Technovit 9100 hardener 2 - component (4)
Hardening liquid 2 acts as a catalyst for hardener 1 to facilitate targeted polymerisation even at very low temperatures [< 0 °C].
Technovit 9100 regulator - component (5)
This is comprised of a reactive organic compound that facilitates a regulated polymerisation with controlled low temperature tops even for large quantities of polymerisation.